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KMID : 0613820040140010180
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Journal of Life Science
2004 Volume.14 No. 1 p.180 ~ p.189
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Antioxidative Role of Geraniin in Lipid Peroxidation of Human LDL
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Ryu Beung-Ho
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Abstract
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Growing evidence indicates that oxidized low density lipoprotein (LDL) may promote atherogenesis. Therefore, inhibition of LDL oxidation may impede this process. The effect of geraniin on the susceptibility of human low density lipoprotein (LDL) to macrophages-induced oxidation was investigated by monitoring a thiobarbiruric acid reactive substrance (TBARS). The antioxidative activity of geraniin was higher than that of α-tocopherol on low density lipoprotein (LDL) oxidation by thiobarbituric acid reactive substance (TBARS). Geraniin inhibited the Cu²+ mediated oxidation of human LDL in a dose dependent manner at concentration of 50 and 100 μg/mL. Geraniin, almost completely inhibited the macrophages mediated LDL oxidation in electrophoretic mobility and conjugate diene of LDL oxidation. Also, geraniin almost completely inhibited O₂- at concentration of 100 μg/mL. The physiological relevance of the antioxidative activity was validated at the cellular level where geraniin inhibited endothelial cell mediated LDL oxidation, When compound with several other antioxidants geraniin showed a high activity equal to natural antioxidants, α-tocopherol and ascorbic acid, and the synthetic antioxidant, probucol. These results indicate that geraniin might play a protective antioxidant effects on LDL, probably affecting both the structural properties of macrophage and endothelial cell for the LDL oxidation.
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KEYWORD
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Low density lipoprotein (LDL), antioxidant, geraniin
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